Analysis Note

ControlHuman fetal skeletal muscle tissue lysate

Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected COX4 in normal human cardiac muscle tissue.

Research Sub CategoryApoptosis - Additional

Anti-COX4 Antibody detects level of COX4 & has been published & validated for use in WB, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytochrome C oxidase subunit 4 (COX4), is one of 13 subunits of cytochrome c oxidase. Cytochrome c oxidase is responsible for catalyzing the last step of electron transfer through the respiratory chain on the mitochondrial inner membrane. COX4 assembles into sub-complexes with other subunits of cytochrome c oxidase and may represent a rate-limiting intermediate.

Immunogen

KLH-conjugated linear peptide corresponding to human COX4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human fetal skeletal muscle tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected COX4 in 10 µg of human fetal skeletal muscle tissue lysate.

Specificity

Other homologies: Rat and mouse (59% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed.
A non-specific band may be observed at ~58 kDa